Composite
NG2

Part:BBa_K2623022

Designed by: Junhong Chen   Group: iGEM18_XMU-China   (2018-10-02)


A non-GFP form of the BBa_K2623021

This part was designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We insert SpyTag to its N-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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